Regulatory

Part:BBa_K1493803:Experience

Designed by: Max van der Ploeg   Group: iGEM14_Wageningen_UR   (2014-10-07)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1493803

User Reviews

The promoter was combined with GFP to check for its functionality. Wageningen_UR_killswitch_Pic13.jpg Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is BBa_K1493801, P2 is BBa_K1493802, P3 is BBa_K1493803, P4 is BBa_K1493804, P5 is BBa_K1493805, P6 is BBa_K1493806.

Figure 1 shows the P3 (promoter with CIλ and LacI operator sites), it is not expressing GFP suggesting that it is either non functional or a very weak promoter.

UNIQa11b84bdcbf47962-partinfo-00000000-QINU UNIQa11b84bdcbf47962-partinfo-00000001-QINU